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Inhibin, beta A (Activin A, activin AB alpha polypeptide) ;Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ;cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ;INHBA ;hCG_17267 , tcag7.474 ;
| 貨號 | EK0301-BV |
|---|---|
| 產品名稱 | Bovine Activin A ELISA Kit |
| 產品描述 | Sandwich High Sensitivity ELISA kit for Quantitative Detection of Bovine Activin A. 96wells/kit, with removable strips. |
| 免疫原 | Expression system for standard: CHO; Immunogen sequence: G311-S426 |
| 交叉反應 | There is no detectable cross-reactivity with other relevant proteins. |
| 敏感性 | <12pg/ml |
| 檢測范圍 | 15.6pg/ml-1000pg/ml |
| 基因名稱 | INHBA |
| 基因全名 | inhibin, beta A |
| Uniprot ID | A4D1W7 |
| 指標別名 | Inhibin, beta A (Activin A, activin AB alpha polypeptide) ;Inhibin, beta A (Activin A, activin AB alpha polypeptide), isoform CRA_a ;cDNA FLJ75379, highly similar to Homo sapiens inhibin, beta A (activin A, activin AB alpha polypeptide), mRNA ;INHBA ;hCG_17267 , tcag7.474 ; |
| 背景資料 | Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules. Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types. Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa. |
| 顯色時間 | 25-30分鐘 |
| SKU | 名稱 | 數量 |
| Catalog number | Description | Quantity |
| EK0301-CAP | 96-well plate precoated with anti- human Activin A antibody | 1 |
| EK0301-ST | Lyophilized recombinant human Activin A standard | 10ng/tube×2 |
| EK0301-DA | Biotinylated anti- human Activin A antibody | 100μl(dilution 1:100) |
| AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
| AR1106-1 | Sample diluent buffer | 30 ml |
| AR1106-2 | Antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
● 1.96孔讀板儀器。
● 2.自動洗板機.
● 3.移液器和槍頭。建議使用多通道移液器/排槍。
● 4.Eppendorf 管。
● 5.額外洗板緩沖液 (neutral PBS 或 TBS。試劑盒內提供足量。如需額外緩沖液可參考如下配方)。
◆ 1. 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
◆ 2. 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*樣品準備的相關試劑也不含在試劑盒中。| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 90 | 281 | 467 | 95 | 293 | 489 |
| Standard deviation | 4.7 | 15.7 | 27.6 | 7.8 | 18.5 | 24.9 |
| CV(%) | 5.2 | 5.6 | 5.9 | 8.2 | 6.3 | 5.1 |
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